Validated RP-HPLC Method Development for Gentamicin Microanalysis in Pharmaceutical Dosage Forms, Urine and Blood

Autor:	Ayaz Ali Memon, M. Y. Khuhawer, M. L. Maheshwari, Naheed Memon, Abdul Hakeem Memon
Rok vydání:	2021
Předmět:	Pharmacology Detection limit Chromatography Chemistry Aminoglycoside Urine medicine.disease High-performance liquid chromatography Microanalysis Dosage form Ototoxicity Drug Discovery medicine Gentamicin medicine.drug
Zdroj:	Pharmaceutical Chemistry Journal. 55:524-530
ISSN:	1573-9031 0091-150X
Popis:	Gentamicin is an important member of the aminoglycoside class of antibiotics having broader spectrum with extend to ototoxicity and nephrotoxcity. A reliable and accurate high performance liquid chromatography (HPLC) procedure was established using Agilent Zorbax Eclipse plus phenyl hexyl C18 (4.6 × 250 mm, 5 μm) column and UV detection at 330 nm. Recovery of gentamicin from biological and pharmaceutical samples was more than 95-98% after spiking the drug. The sensitivity of method is predicted by calculating a lower level of the limit of detection and lower limit of quantification (0.286 and 0.867 μg/mL, respectively. The procedure was used to quantify gentamicin in biological fluids and pharmaceuticals dosage form.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::e20e59012f311683f302a9e2a22cacf7 https://doi.org/10.1007/s11094-021-02451-8 Zobrazit plný text záznamu Plný text ve formátu PDF