Reductive Dechlorination of 1,2-dichloroethane to Ethylene by Anaerobic Enrichment Culture Containing Vulcanibacillus spp
| Autor: | Nipa Milintawisamai, Utumporn Ngivprom, Alissara Reungsang |
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| Rok vydání: | 2021 |
| Předmět: | |
| Zdroj: | Walailak Journal of Science and Technology (WJST). 18 |
| ISSN: | 2228-835X 1686-3933 |
| Popis: | Bioremediation has been widely used for clean-up of 1,2-dichloroethane (DCA) at contaminated sites. Only a small number of specific anaerobes, halorespiring bacteria (HRB), have been reported to degrade DCA. The goals of this research were the screening and isolation of HRB with capable dechlorination of DCA. HRB were screened and isolated from 7 enrichment cultures (ES1-ES7), from DCA-contaminated soils, on bicarbonate-buffered basal salts medium containing 10 mM acetate and 250 µM DCA under anaerobic conditions and analyzed by gas chromatography. The results showed that the mixed cultures of ES3 and ES5 could reductively dechlorinate DCA to ethylene via a direct reductive dihaloelimination pathway. In particular, ES5 showed rapid transformation of 250 µM DCA to ethylene within 6 days at 30 °C. Specific microbial populations of Vulcanibacillus spp., elucidated by polymerase chain reaction-denaturing gradient gel electrophoresis, were found only in ES3 and ES5, which was related to reductive dihaloelimination activities on DCA. A 16S rRNA gene analysis of isolate es5d8 from mixed culture ES5 revealed 2 strains of Vulcanibacillus spp. (KKU-DCA1 and KKU-DCA2) concerned with dechlorinating DCA. These findings suggested that Vulcanibacillus spp. were HRB that have the potential for detoxifying DCA. |
| Databáze: | OpenAIRE |
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