High Speed HPLC Analysis of Polyamines in Plant Tissues

Autor:	Hugo J.-P. Walter, Jan M. C. Geuns
Rok vydání:	1987
Předmět:	Hplc analysis Chromatography Physiology Spermine Plant Science High-performance liquid chromatography Spermidine Micrometre chemistry.chemical_compound chemistry Volume (thermodynamics) Genetics Putrescine Acetonitrile
Zdroj:	Plant Physiology. 83:232-234
ISSN:	1532-2548 0032-0889
DOI:	10.1104/pp.83.2.232
Popis:	A high speed high performance liquid chromatography (HPLC) method for the quantification of putrescine, spermidine, and spermine in biological samples is described. The dansylation is followed by a sample cleanup. The isocratic HPLC-analysis with acetonitrile:H2O (72:28 volume/volume) on 10 centimeter long, 3 micrometer octadecyl silica columns (3 millimeter inner diameter) takes only 4.5 minutes. By our method about 100 analyses can be done in 1 day.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::e1ea90e327dec4e76b7898da87037675 https://doi.org/10.1104/pp.83.2.232 Zobrazit plný text záznamu