Identification of Barnacle Shell Proteins by Transcriptome and Proteomic Approaches
| Autor: | Keiju Okano, Mieko Oguro-Okano, Yasuyuki Nogata, Wei-Pang Zhang, Pei-Yuan Qian, Noriaki Ozaki, Benny K. K. Chan, Jin Sun, Hsiu-Chin Lin, Yue Him Wong, Erina Yoshimura |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
030102 biochemistry & molecular biology Megabalanus rosa Shell (structure) Arthropod cuticle Biology Proteomics biology.organism_classification Transcriptome 03 medical and health sciences Barnacle 030104 developmental biology Secretory protein Biochemistry Identification (biology) |
| Zdroj: | Biomineralization ISBN: 9789811310010 |
| DOI: | 10.1007/978-981-13-1002-7_11 |
| Popis: | In barnacle shell, the calcified shell layer is laid on top of the epicuticle. Here, we report our strategy and some preliminary results on the identification of potential shell proteins of the barnacle Megabalanus rosa. At first, M. rosa proteins from acid-soluble and acid-insoluble shell extracts were subjected to proteomic analysis and searched against M. rosa complete transcriptome. Then using the information that the calcified shell is formed just after the larval-adult molt, juvenile differentially expressed genes against larval stages were screened. Sixty secretory protein sequences were identified as primary candidates of M. rosa shell proteins, among which 37 are novel proteins. |
| Databáze: | OpenAIRE |
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