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Aim The Luminex Single Antigen Assay is an integral part of the transplant program to determine patient antibodies. It has an important clinical impact on transplant outcomes. It is suggested that the Luminex single antigen testing on heart transplant patients often has a high mean fluorescent intensity (MFI) of the negative control bead (NC), which makes antibody interpretation difficult. This study aims to determine if airfuge is useful to reduce this background. Methods HLA class I and class II single antigen testing was performed on 20 serum samples from pre- or post-heart transplant patients. All samples were tested in parallel with and without airfuge. Out of 20 samples, 10 samples were randomly selected from our daily heart patient worklist. Another 10 samples exhibited a high background in the original assay. The valid range for the negative control bead cutoff was defined as 0–1000 in our laboratory. Results MFI of NC in single antigen testing on the randomly selected samples were all below 200. MFI of NC in one sample increased from 100 to 250 after airfuge. For the group of samples that originally displayed high background, airfuge treatment decreased MFI of NC 2 folds in only 7, out of 20, tests. Of these 7 tests, NC in 5 tests that had MFI > 5000 without airfuge, decreased to around 2000 after airfuge. However, NC beads in 4 tests having MFI Conclusions High NC MFI was not observed in the majority of testing on heart patients. Treatment of sera with airfuge in heart patient samples before testing has a significant effect in decreasing NC value only for some high background sera. It is not effective in most patients’ sera. It is unnecessary to pretreat sera with airfuge for single antigen testing for heart transplant patients. |
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