Autor: |
Richter, Antje, Kiehl, Steffen, Köger, Nicole, Breuer, Janina, Stiewe, Thorsten, Dammann, Reinhard |
Rok vydání: |
2017 |
Předmět: |
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DOI: |
10.6084/m9.figshare.c.3794611_d4 |
Popis: |
ZAR1 overexpression blocks cell cycle progression of cancer cell lines. Cell lines HeLa, A549 and HCT116 were transfected with EGFP or ZAR1-EGFP, isolated after 24 h and fixed with ethanol. DNA content was measured by FACS CantoII using propidium iodide staining to determine cell cycle arrest. S phase alteration is marked in red. A–C Flow cytometry gating is shown for HeLa, A549 and HCT116 cancer cells and D shows quantification for HCT116. (PDF 345 kb) |
Databáze: |
OpenAIRE |
Externí odkaz: |
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