Effect of Surfactants in Soybean Lecithin Liposomes Studied by Energy Transfer Between NBD-PE and N-Rh-PE
| Autor: | J. I. N. Rocha Gomes, Paulo J. G. Coutinho, M. E. C. D. Real Oliveira, Adelina L. F. Baptista |
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| Rok vydání: | 2000 |
| Předmět: |
Sodium laurate
Phosphatidylethanolamine 010407 polymers Liposome Chromatography Vesicle technology industry and agriculture Pharmaceutical Science Ether 02 engineering and technology 021001 nanoscience & nanotechnology 7. Clean energy 01 natural sciences Micelle 0104 chemical sciences Rhodamine chemistry.chemical_compound chemistry Rhodamine B lipids (amino acids peptides and proteins) 0210 nano-technology Nuclear chemistry |
| Zdroj: | Journal of Liposome Research. 10:419-429 |
| ISSN: | 1532-2394 0898-2104 |
| DOI: | 10.3109/08982100009031108 |
| Popis: | The effect of C12E8 (polyoxyethylene 8 lauryl ether) and sodium laurate on the structural properties of soybean lecithin vesicles was studied in different concentrations of surfactants, using the fluorescent probes NBD-PE (N-7-nitro-2,1,3-benzodiazoyl phosphatidylethanolamine) and N-Rh-PE (N-lissamine rhodamine B sulfonyl phosphatidylethanolamine). Direct energy transfer studies were carried out in labelled vesicles with addition of surfactants. Rhodamine emission with maximum at β585 nm was detected by excitation of NBD at λ = 473 nm. This fact is caused by direct energy transfer process from NBD to rhodamine. The yield of this process decreases with increasing amounts of surfactants, indicating that the average spatial separation of the lipid probes increases, as a result of the enlargement of the vesicle size and due to alteration of its structure to mixed micelles. |
| Databáze: | OpenAIRE |
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