Graphene Doped Mn2 O3 Nanofibers as a Facile Electroanalytical DNA Point Mutation Detection Platform for Early Diagnosis of Breast/Ovarian Cancer

Autor:	Avss Narayana Rao, Shiv Govind Singh, Suryasnata Tripathy, Rahul Gangwar, Patta Supraja, Siva Rama Krishna Vanjari
Rok vydání:	2018
Předmět:	Materials science Graphene Point mutation 010401 analytical chemistry Mutant Nanotechnology 02 engineering and technology Glassy carbon 021001 nanoscience & nanotechnology 01 natural sciences 0104 chemical sciences Analytical Chemistry law.invention chemistry.chemical_compound chemistry law Nanofiber Mutation (genetic algorithm) Electrochemistry Differential pulse voltammetry 0210 nano-technology DNA
Zdroj:	Electroanalysis. 30:2110-2120
ISSN:	1040-0397
DOI:	10.1002/elan.201800220
Popis:	This paper demonstrates a simple, label‐free detection methodology for detecting single point DNA mutations. Single point mutation detection is a key enabler for diagnosis and prevention of several genetic disorders that manifest into cancers. Specifically for this purpose, herein, an electrochemical biosensor utilizing electrospun graphene doped manganese III oxide nanofibers (GMnO) is developed. The charge transfer resistance offered by GMnO is extremely sensitive to the localized change in the conductivity. This sensitivity, attributed to the low band gap of Mn2O3 and high charge transfer kinetics of graphene, is explored in the proposed mutation detection platform. As a proof of concept, ultrasensitive detection of BRCA1 gene specific point mutation is demonstrated. The target specific single stranded probe DNA is immobilized onto GMnO modified glassy carbon working electrodes via chemisorption. Post target‐DNA hybridization, differential pulse voltammetry is employed to facilitate detection of targeted point mutation, wherein, difference in peak currents is used to distinguish the target DNA as normal or mutant. Efficiency of the proposed method is evaluated against a target concentration ranging from 10 pM−1 μM. With respect to the mutated target DNA, the LoD of the proposed device is found to be 0.8±0.069 pM. The proposed approach can be extended for detecting any mutation/hybridization of interest by simply adapting an appropriate functionalization protocol.
Databáze:	OpenAIRE
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