| Autor: |
Andre I. Salama, Charles F. Saller, Lillian Lin |
| Rok vydání: |
1985 |
| Předmět: |
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| Zdroj: |
Journal of Chromatography B: Biomedical Sciences and Applications. 341:43-51 |
| ISSN: |
0378-4347 |
| DOI: |
10.1016/s0378-4347(00)84008-6 |
| Popis: |
A rapid and sensitive automated system for measuring cyclic adenosine 3′,5′-monophosphate (cAMP) synthesized from either radiolabelled adenine or adenosine 5′-triphosphate (ATP) in intact and broken cell tissue preparations, respectively, is described. After incubation with radiolabelled precursor, tissue samples are deproteinized and then injected directly onto a reversed-phase high-performance liquid chromatographic column. The column effluent fraction which contains cAMP is collected into scintillation vials and assayed for tritium by liquid scintillation spectrometry. Since the high-performance liquid chromatographic and fraction collection procedures are automated, over fifty samples can be analyzed in duplicate in a single day. The utility of this assay is illustrated by investigations of the effects of β-adrenergic receptor stimulation on cAMP synthesis in tissue slices prepared from rat cerebral cortex and dopamine on cAMP synthesis in striatal membrane preparations. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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