Physiological and biochemical aspects of diversity of Bradyrhizobium sp. (Vigna) from three West African soils

Autor: M. D. Stowers, M.L. Maina, A. Ayanaba, Allan R. J. Eaglesham, Martha J. Sinclair, B. J. Goldman
Rok vydání: 1987
Předmět:
Zdroj: Soil Biology and Biochemistry. 19:575-581
ISSN: 0038-0717
DOI: 10.1016/0038-0717(87)90101-5
Popis: Bradyrhizobial isolates from the nodules of cowpeas [ Vigna unguiculata (L.) Walp.] grown at three climatically-distinct West African locations (Ibadan and Onne in Nigeria and Maradi in Niger) were examined for physiological and biochemical characteristics to gain an understanding of the ranges of diversity of types within and between soil populations. Patterns of utilization of 20 carbohydrates and three vitamins showed similarities between locations and to other bradyrhizobia reported previously. Strains from Maradi, in the sahel-savanna, had the greatest tolerance to high temperature. Different proportions of two colony types, designed “wet” and “dry”, were found at each location. Strains with the “wet” colony morphology were tolerant of 0.5% NaCl whereas “dry” strains were not. Production of a chlorosis-inducing toxin in broth culture was more prevalent among “dry” strains. Protein-band patterns on SDS-polyacrylamide gels indicated that the “wet” and “dry” types are fundamentally different.
Databáze: OpenAIRE