Single enzyme nanoparticles armored by a thin silicate network: Single enzyme caged nanoparticles
| Autor: | Hansol Kim, Jungbae Kim, Ho Nam Chang, Seong H. Kim, Byung-Gee Kim, Hyon Bin Na, Jay W. Grate, Byoung Chan Kim, Erik Hsiao, Seung wook Chung, Byoungsoo Lee, Jongkyu Youn, Jinwoo Lee, Taeghwan Hyeon, Sung Gil Hong, Changwon Lee, Jonathan S. Dordick, Hyun Gyu Park |
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| Rok vydání: | 2017 |
| Předmět: |
chemistry.chemical_classification
Immobilized enzyme biology General Chemical Engineering Biomolecule Inorganic chemistry Nanoparticle 02 engineering and technology General Chemistry Mesoporous silica 010402 general chemistry 021001 nanoscience & nanotechnology 01 natural sciences Industrial and Manufacturing Engineering Silicate Enzyme assay 0104 chemical sciences chemistry.chemical_compound chemistry Chemical engineering Polymerization biology.protein Environmental Chemistry 0210 nano-technology Biosensor |
| Zdroj: | Chemical Engineering Journal. 322:510-515 |
| ISSN: | 1385-8947 |
| Popis: | For the encapsulation of biomolecules in inorganic materials, we have developed a unique enzyme-silicate conjugate material that consists of a self-assembled molecularly thin silicate layer on the surface of each individual enzyme molecule. The enzyme-silicate conjugate materials, called single enzyme caged nanoparticles (SECNs), were synthesized via the silica polymerization on the surface of enzyme molecule after solubilizing each enzyme molecule in hexane by using a tiny amount of surfactant, called “ion-pairing”. SECNs possess near native enzyme activity in aqueous media with minimal substrate diffusional limitations, and are highly stable under the protection of silicate network cage. Due to their nearly molecular size, SECNs can also be adsorbed into mesoporous silica materials to yield robust and easily-recyclable enzymatic systems that can be used in a number of potential biocatalytic applications such as diagnostics, biosensors, biotransformations, biofuel production, bioremediation and CO2 capture. |
| Databáze: | OpenAIRE |
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