Detection of the F129L mutation in the cytochromebgene inPhakopsora pachyrhizi
| Autor: | Gerd Stammler, Louise Larissa May De Mio, Ana C Klosowski, Ronaldo Rodrigues, Simone Miessner |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
0106 biological sciences
Genetics biology Cytochrome b Point mutation Mutant General Medicine biology.organism_classification 01 natural sciences Fungicide 010602 entomology Insect Science Phakopsora pachyrhizi Mutation (genetic algorithm) Pyrosequencing Agronomy and Crop Science Gene 010606 plant biology & botany |
| Zdroj: | Pest Management Science. 72:1211-1215 |
| ISSN: | 1526-498X |
| DOI: | 10.1002/ps.4099 |
| Popis: | BACKGROUND Asian soybean rust, caused by Phakopsora pachyrhizi, is mostly controlled by demethylation inhibitor (DMI) and quinone outside inhibitor (QoI) fungicides. Mutations in the cytochrome b (CYTB) gene can lead to pathogen resistance to QoIs. The occurrence of the mutations in codons 129, 137 and 143 in the CYTB gene was investigated, and a pyrosequencing assay was developed for rapid and quantitative detection of the F129L mutation. RESULTS Molecular analysis of the CYTB gene showed the presence of the F129L mutation in field samples and monouredinial isolates, while other mutations (G143A and G137R) were not found. The pyrosequencing was an effective method for quantitative detection of the F129L mutation, and many of the P. pachyrhizi samples showed high frequency of F129L. CONCLUSION This is the first report of the occurrence of the F129L mutation in P. pachyrhizi. The practical relevance of this mutation for field efficacy of QoIs needs further investigation. © 2015 Society of Chemical Industry |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text