Liophilic Mobile Phase Additives in Reversed Phase HPLC

Autor: Alexey A. Makarov, Rosario LoBrutto, Yuri Kazakevich
Rok vydání: 2008
Předmět:
Zdroj: Journal of Liquid Chromatography & Related Technologies. 31:1533-1567
ISSN: 1520-572X
1082-6076
DOI: 10.1080/10826070802125918
Popis: The separation of basic compounds can be challenging and the use of inorganic mobile phase additives have been successfully used in chromatographic methods development. About fifteen years ago the role of these additives as ion-interaction agents for selectively adjusting retention of ionic analytes was discovered and the theory of chaotropicity was applied to reversed phase chromatography. In the last ten years the studies of the influence of these counterions on the retention of ionizable analytes and the interaction with the stationary phase in various hydro-organic eluents has expanded our knowledge of this phenomenon. The general view and understanding of the process have been significantly updated and the use of these ionic additives (liophilic ions) in the mobile phase has become regular practice in the pharmaceutical industry for optimization and fine tuning of complex separations. This paper reviews the latest developments in the field and discusses the modification and expansion of our ...
Databáze: OpenAIRE
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