Internal substrate concentrations during biotransformation of octanoic acid into 2-heptanone by spores ofPenicillium roquefortii

Autor:	J. B. Gros, Christian Larroche, I. Besson
Rok vydání:	1996
Předmět:	chemistry.chemical_classification Chromatography Stereochemistry Fatty acid Bioengineering Penetration (firestop) Biology Applied Microbiology and Biotechnology Spore chemistry.chemical_compound Membrane chemistry Biotransformation 2-Heptanone Efflux Stoichiometry Biotechnology
Zdroj:	Journal of Industrial Microbiology. 16:29-35
ISSN:	1476-5535 0169-4146
Popis:	Internal substrate concentrations were monitored during biotransformation of octanoic acid into 2-heptanone by spores ofPenicillium roquefortii. The fatty acid rapidly enters the spores in its undissociated form, and a Collander-type relation shows that it strongly accumulates in the spore wall and membrane; this accumulation is reversible. The reaction takes place with cytoplasmic substrate concentrations that quickly fall to zero, and the process is limited by octanoic acid penetration into the cells. This entry is accompanied by proton efflux and involves an active transport process with a H+-ATPase system that exhibits Michaelian behavior. The driving force is postulated to be ΔpH, which takes a value set by the initial substrate concentration through the stoichiometry of the H+/octanoic acid exchange.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::e17d7ed8c4dbce6dd0805ef20fe22fe4 https://doi.org/10.1007/bf01569918 Zobrazit plný text záznamu Full text from SpringerLink