Production of arachidonic acid by filamentous fungus,Mortierella alliaceastrain YN-15
| Autor: | Yoshio Tanaka, Kazuhisa Ono, Tsutomu Morinaga, Osamu Suzuki, Seiko Shigeta, Tsunehiro Aki, Kengo Akimoto, Katsuyuki Ishihara, Yumiko Nagahata, Kenichi Higashiyama, Shigeaki Fujikawa, Seiji Kawamoto |
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| Rok vydání: | 2001 |
| Předmět: |
chemistry.chemical_classification
food.ingredient biology Starch General Chemical Engineering Organic Chemistry Fatty acid biology.organism_classification Soybean oil Oleic acid chemistry.chemical_compound food chemistry Biochemistry Yeast extract Arachidonic acid Mortierella Polyunsaturated fatty acid |
| Zdroj: | Journal of the American Oil Chemists' Society. 78:599-604 |
| ISSN: | 0003-021X |
| DOI: | 10.1007/s11746-001-0311-2 |
| Popis: | A filamentous fungus producing significant levels of arachidonic acid (AA, C20∶4n−6) was isolated from a freshwater pond sample and assigned to the species Mortierella alliacea. This strain, YN-15, accumulated AA mainly in the form of triglyceride in its mycelia. An optimized culture in 25 L of medium containing 12% glucose and 3% yeast extract yielded 46.1 g/L dry cell weight, 19.5 g/L total fatty acid, and 7.1 g/L AA by 7-d cultivation in a 50-L jar fermenter. Assimilation of soluble starch by YN-15 was notably enhanced by the addition of oleic acid, soybean oil, ammonium sulfate, or potassium phosphate to a starch-based medium. Using starch as a main carbon source in the pre-pilot scale cultivation improved the production of AA by up to 5.0 g/L. Mortierella alliacea strain YN-15 is therefore a promising fungal isolate for industrial production of AA and other polyunsaturated fatty acids. |
| Databáze: | OpenAIRE |
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