Measurement of estriol in plasma by gas-liquid chromatography
| Autor: | Tzu Y. Lee, Adolf E. Schindler, Walter L. Herrmann, Vannee Ratanasopa |
|---|---|
| Rok vydání: | 1967 |
| Předmět: |
medicine.medical_specialty
Pregnancy Eclampsia business.industry Urinary system Obstetrics and Gynecology Estriol medicine.disease Endocrinology Internal medicine Diabetes mellitus medicine Gestation Protein precipitation Gas chromatography business hormones hormone substitutes and hormone antagonists reproductive and urinary physiology |
| Zdroj: | American Journal of Obstetrics and Gynecology. 99:295-302 |
| ISSN: | 0002-9378 |
| DOI: | 10.1016/s0002-9378(16)34533-1 |
| Popis: | A method for the measurement of plasma estriol in pregnancy is described, using a radioactive internal standard, acid hydrolysis, phenolic extraction, thin-layer chromatography, and separation and quantification of estriol TMS derivative by gas-liquid chromatography (GLC). Accuracy, sensitivity, specificity, and precision were found to be acceptable. Plasma estriol in normal pregnancy increased from 25 weeks’ gestation to term from 0.5 μg to 3 μg up to 9 μg to 22 μg per 100 ml. In pregnancy complicated by severe pre-eclampsia, diabetes mellitus, fetal death, and eclampsia, low values of estriol in plasma were found. No correlation was found between plasma estriol and severity of Rh disease. Increase or decrease of plasma and urinary estriol levels in pregnancy correlated well. It was demonstrated that protein precipitation caused a decrease of detectable plasma estriol by 40.3 ± 8.8 per cent. |
| Databáze: | OpenAIRE |
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