Auxin Starvation Results in G2-Arrest in Suspension-Cultured Tobacco Cells

Autor: Jan W. Kijne, Kees R. Libbenga, F.T. Nicoloso, M. Harteveld, K.B. Koens
Rok vydání: 1995
Předmět:
Zdroj: Journal of Plant Physiology. 147:391-396
ISSN: 0176-1617
DOI: 10.1016/s0176-1617(11)82173-5
Popis: Summary In cell suspensions of tobacco LT cells, cultured on Linsmaier and Skoog medium with 2,4-dichlorophenoxyacetic acid (2,4-D) as the sole plant growth regulator, final cell number is dependent on the initial concentration of 2,4-D in the medium. At low concentrations of 2,4-D, accumulation of cells in the G2-phase of the cell cycle appeared to increase. Cells arrested in the G2-phase could enter mitosis and continue cycling after addition of 2,4-D or the auxin analogue NAA. Addition of cytokinins or 3,5-D did not enable pasage of the cells into mitosis. This suggests that auxin (analogues) influence a restriction point in the G2-phase of the cell cycle in tobacco LT cells.
Databáze: OpenAIRE