Morphgenomics based identification of Fusarium proliferatum causing Syagrus romanzoffiana wilt and exploitation of antifungal potential of Trichoderma species against this pathogen

Autor: Nabeeha Aslam Khan, Imran Ul Haq, Fathia Mubeen, Rashad Qadri, Amer Habib, Siddra Ijaz, Anjum Faraz
Rok vydání: 2020
Předmět:
Zdroj: Journal of Plant Pathology. 102:1097-1105
ISSN: 2239-7264
1125-4653
Popis: Chlorosis on rachis followed by plant wilting of Syagrus romanzoffiana (Queen Palm) was observed during the survey of commercial ornamental plant growing nurseries and landscapes of sixteen locations from five districts of Punjab province. About 25% plants were found affected by the disease. Symptomatic root and leaf tissues were sliced, surface sterilized, transferred on PDA medium and incubated at 26 °C. Frequently whitish to purple fungal colony pigmentation was observed with club shaped microconidia and slender shaped macroconidia. Based on morpho-cultural characters the fungal isolate was identified as Fusarium proliferatum. The internal transcribed spacer (ITS) region and translation elongation factor 1-α (TEF1-α) region were sequenced for molecular characterization. Phylogenetic trees were constructed based on the sequences of both investigated loci to explore the place of investigated fungal isolate FMB-F-P-QP in evolutionary hierarchy of Fusarium species. In phylograms, this isolate was clearly nested with the strains of Fusarium proliferatum. The morphogenomic studies proved this isolate as F. proliferatum. The characterized culture deposited in FMB-Culture Collection-UAF (FMB 0133) and generated sequences of both loci were submitted to GenBank (accession No. MH557215 for ITS; accession No. MH568689 for TEF1-α). Koch’s postulates were accomplished by applying F. proliferatum and yellowing of leaves started three weeks post inoculation while chlorosis on one side of rachis or complete wilting appeared after six months. This is the first reported study of Syagrus romanzoffiana wilt caused by F. proliferatum in Pakistan. Four strains of three Trichoderma species (one strain of T. harzianum, two strains of T. atroviridae and one strain of T. viridae) were evaluated for their antagonistic potential against F. proliferatum. T. harzianum showed higher antifungal potential 85%, as compared to the other tested strains.
Databáze: OpenAIRE
Externí odkaz: