| Autor: |
C. Schneider, R. Brodt, H. D. Brede, V. Brade, E. B. Helm, H. Rübsamen-Waigmann, U. Thums |
| Rok vydání: |
1994 |
| Předmět: |
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| Zdroj: |
Methods in DNA Amplification ISBN: 9780306449086 |
| Popis: |
Various polymerase chain reaction (PCR) assays have been described for the rapid identification of mycobacteria in clinical specimens. To assess the value of the assays in routine laboratory work the results obtained by PCR were compared with those obtained by standard microbiological methods and clinical data. 56 sputum specimens were collected for investigation of mycobacterial infection in HIV-infected patients, 73 from patients without HIV-infection. In a substantial number of HIV patients with tuberculosis both chest x-ray and the clinical course were found to be unusual, in concordance with published data. Rapid detection of mycobacteria by laboratory methods is therefore of great importance for the care of HIV-infected patients. Isolation of mycobacteria from clinical samples takes up to 8 weeks for culture on solid medium and up to 20 days by the BACTEC system, which is based on the measurement of carbon dioxide released by bacteria during growth in liquid medium. It has often been reported that amplification of mycobacterial DNA by use of the polymerase chain reaction (PCR) technique permits rapid detection of mycobacteria directly in clinical samples. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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