ZxABCG11 from the xerophyte Zygophyllum xanthoxylum enhances drought tolerance in Arabidopsis thaliana through modulating cuticular wax accumulation
| Autor: | Zhang Jing, Wan-Peng Bai, Ai-Ke Bao, Lin-Bo Liu, Hai-Shuang Liu, Suo-Min Wang, Ye Tian, Owen Rowland, Hu-Jun Li, Timothy M. Garant, Hui-Jun Yuan |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
0106 biological sciences
0301 basic medicine Wax biology Chemistry Cuticle fungi Drought tolerance food and beverages Plant Science Genetically modified crops biology.organism_classification 01 natural sciences Cell wall 03 medical and health sciences 030104 developmental biology Xerophyte visual_art Arabidopsis Botany visual_art.visual_art_medium Arabidopsis thaliana Agronomy and Crop Science Ecology Evolution Behavior and Systematics 010606 plant biology & botany |
| Zdroj: | Environmental and Experimental Botany. 190:104570 |
| ISSN: | 0098-8472 |
| DOI: | 10.1016/j.envexpbot.2021.104570 |
| Popis: | Cuticular waxes, a waterproofing hydrophobic barrier that coats the aerial surfaces of all terrestrial plants, protects plants from various environmental stresses, particularly drought stress. The molecular basis of cuticular wax deposition in xerophytes (e.g. desert plants), including transport mechanisms of waxes to the cell wall, is poorly understood. Here, ZxABCG11 encoding an ATP binding cassette (ABC) transporter was isolated from the xerophyte Zygophyllum xanthoxylum, since we found it was predominantly expressed in young leaves and induced by salt, drought, heat, and combined drought and heat stresses. To ascertain the function of ZxABCG11, we introduced ZxABCG11 into Arabidopsis thaliana and found an increased abundance of cuticular wax on stems and leaves of the transgenic plants, which also exhibited a thickened cuticle and reduced cuticle permeability. Transcript expression analysis revealed that many genes related to wax biosynthesis and transport were up-regulated in these ZxABCG11 transgenic plants. Furthermore, compared with wild type Arabidopsis, ZxABCG11 transgenic plants displayed a higher biomass and survival rate under drought conditions. Taken together, we propose that the expression of ZxABCG11 in Arabidopsis promotes cuticular wax accumulation and thicker cuticle formation, thereby enhancing drought tolerance. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect