Geotrichum candidum 4013: Extracellular lipase versus cell-bound lipase from the single strain

Autor:	Klara Hlavsova, Zdeněk Wimmer, Martina Mackova, Marie Zarevúcka, Helena Sovová
Rok vydání:	2009
Předmět:	chemistry.chemical_classification Chromatography biology Chemistry Process Chemistry and Technology Triacylglycerol lipase Bioengineering Geotrichum biology.organism_classification Biochemistry Catalysis Enzyme assay Hydrolysis Enzyme biology.protein Extracellular Lipase Polyunsaturated fatty acid
Zdroj:	Journal of Molecular Catalysis B: Enzymatic. 61:188-193
ISSN:	1381-1177
DOI:	10.1016/j.molcatb.2009.06.012
Popis:	Two types of lipases (extracellular and cell-bound) were produced by Geotrichum candidum 4013 in liquid medium and were used as biocatalysts in blackcurrant oil hydrolysis. Reaction products were analysed for the degree of conversion from which enzyme activity was evaluated, and the composition of free fatty acids was compared to the composition of oil substrate. The enzyme activity was measured also before and after the reaction in SC-CO 2 . The fatty acid composition of the acids liberated from oil by hydrolysis suggests a specificity of the cell-bound and extracellular enzymes from Geotrichum candidum 4013. The extracellular lipase displays low selectivity to the polyunsaturated fatty acids, and the cell-bound lipase possesses selectivity to the saturated fatty acids. Enantioselectivity of the tested processes achieved with both induced enzymes was high (from 43 to 242). The activity of all enzymes has markedly increased after their exposure to SC-CO 2 . The treatment of enzymes by SC-CO 2 could be easy-to-use approaches to improve the efficiency of enzymatic reactions.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::e127edf22285777fb2a824071208f3e2 https://doi.org/10.1016/j.molcatb.2009.06.012 Zobrazit plný text záznamu Full Text from ScienceDirect