Isolation of the tyrosine phenol lyase gene from Citrobacter freundii
Autor: | Henry Brzeski, J. Polak |
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Rok vydání: | 1990 |
Předmět: |
biology
Bioengineering Tyrosine phenol-lyase General Medicine biochemical phenomena metabolism and nutrition Molecular cloning biology.organism_classification Applied Microbiology and Biotechnology Molecular biology Restriction fragment Citrobacter freundii Restriction enzyme genomic DNA Biochemistry biology.protein Cosmid bacteria Tyrosine Biotechnology |
Zdroj: | Biotechnology Letters. 12:805-810 |
ISSN: | 1573-6776 0141-5492 |
DOI: | 10.1007/bf01022599 |
Popis: | The gene for the enzyme tyrosine phenol-lyase (TPL) was initially isolated on a 45 kbp fragment of Citrobacter freundii genomic DNA contained in a cosmid. Subsequent restriction enzyme digestion and sub-cloning resulted in the gene being contained on a 2.4 kbp DNA fragment. |
Databáze: | OpenAIRE |
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