Isolation of biosurfactant-producing bacteria, product characterization, and evaluation

Autor:	Ibrahim M. Banat, S Haditirto, AS Aburuwaida, M. Kadri, Ahmed M. Salem
Rok vydání:	1991
Předmět:	chemistry.chemical_classification Chromatography biology Chemistry Residual oil Primary metabolite Bioengineering biology.organism_classification Applied Microbiology and Biotechnology Micelle Dilution Hydrocarbon Pulmonary surfactant Rhodococcus Bacteria Biotechnology
Zdroj:	Acta Biotechnologica. 11:315-324
ISSN:	1521-3846 0138-4988
DOI:	10.1002/abio.370110405
Popis:	A gram-positive, nonfermentative, rod-shaped bacterium designated St-5, identified as Rhodococcus, was isolated from Kuwait soil. Grown on hydrocarbon, such as kerosene and n-paraffin, the bacterium produced surface-active compounds (biosurfactants). Measurements of surface tension, critical micelle dilution and emulsifying activity indicated that the biosurfactant is produced as a primary metabolite. The ST-5 culture surface-active component is mainly glycolipid in nature. Whole-culture broth dropped surface tension to values below 27 mN/m and was stable during, exposure to high salinity (10% NaCl), elevated temperatures (120-degrees-C for 15 min) and a wide range of pH values. The culture broth was effective in recovering up to 86% of the residual oil from oil-saturated sand packs, indicating potential value in enhanced oil-recovery processes.
Databáze:	OpenAIRE
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