Transfection optimisation in HEK-293 cell line

Autor:	Muhammad Aurang Zeb, Abu Saim Al Salauddin, Jay Prakash Sah, Deluwer Hossain, Saidin Saclain, Rayhan Chowdhury, M. Abu Sayed, Sayfullah, M Salahuddin, Mahjabin Rashid, Mizanur Rahman Washim, MA Momin
Rok vydání:	2015
Předmět:	General Engineering Transfection Biology HEK 293 Cell Line Molecular biology Protein expression Cell biology chemistry.chemical_compound Plasmid dna chemistry Cell culture Gene expression Incubation DNA
Zdroj:	Asian Journal of Medical and Biological Research. 1:121-129
ISSN:	2412-5571 2411-4472
Popis:	Transfection is the most popular experiments among biological scientist to study the gene expression. But it is really tedious to find out the optimum conditions for protein expression for transfection while working with plasmid DNA. In our present study, the main objective was to setup suitable conditions to study the protein expression. From different conditions, protein expression was observed for 2?g of DNA. We tried different cell number and time frame and got 6.25×105cell/ well of 6 well and 24 hour incubation were the best condition for maximum expression of protein. In our experiment 1:3 ratio of DNA: Lipfectamine was the best for optimum expression of protein in mammalian cell line.Asian J. Med. Biol. Res. June 2015, 1(1): 121-129
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::e0fca18637f9f0985b3a79dab8f89d2f https://doi.org/10.3329/ajmbr.v1i1.25508 Zobrazit plný text záznamu