Salicylic Acid and H2O2 Induce PPO Derived GUS Expression in Arabidopsis
| Autor: | D. Saleem, Wasim Akhtar, Z. Zuhra, Tariq Mahmood, H. Koiwa |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
0301 basic medicine chemistry.chemical_classification Reporter gene biology food and beverages Plant physiology Plant Science biology.organism_classification 01 natural sciences Molecular biology 03 medical and health sciences chemistry.chemical_compound 030104 developmental biology Enzyme chemistry Polyphenol Arabidopsis Plant defense against herbivory Hydrogen peroxide Salicylic acid 010606 plant biology & botany |
| Zdroj: | Russian Journal of Plant Physiology. 67:822-826 |
| ISSN: | 1608-3407 1021-4437 |
| DOI: | 10.1134/s1021443720050131 |
| Popis: | Polyphenol oxidases (PPOs) are ubiquitous enzymes of plant defense which transform polyphenols into quinones that response to biotic and abiotic stresses. Transgenic Arabidopsis thaliana (L.) Heynh. lines harboring OsPPO promoter fused to β-glucoronidase (GUS) reporter gene, were treated exogenously with 0.1, 0.5 and 1 mM concentrations of salicylic acid (SA) and 5, 10 and 15 mM of hydrogen peroxide (H2O2). Plant samples were harvested after 1, 3 and 5 days of treatment. Transcriptional profiling of OsPPOGUS by qRT-PCR revealed the highest 12.96-fold induction with 0.5 mM SA after 3 days of treatment and the lowest fold induction (0.57) with 1 mM SA after one day. With H2O2, the OsPPOGUS mRNA transcript showed the highest accumulation of 21.08-fold change with 10 mM after 5 days of treatment and the lowest induction (3-fold) after 1 day of treatment by 15 mM H2O2. This specific OsPPO induction in response to SA and H2O2 clearly links OsPPO in potential responses to such stressors. |
| Databáze: | OpenAIRE |
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