Evidence for neurotrophic factors associating with TRPV1 gene expression in the inflamed human esophagus
| Autor: | Kun-Ruey Shieh, H. C. Ho, H. T. Hsieh, H. L. Tseng, Chien-Lin Chen, T. T. Liu, Chih-Hsun Yi |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
medicine.medical_specialty
Endocrine and Autonomic Systems Physiology Gastroenterology TRPV1 Biology medicine.disease Reverse transcription polymerase chain reaction Nerve growth factor Endocrinology Real-time polymerase chain reaction nervous system Neurotrophic factors Internal medicine GERD medicine Glial cell line-derived neurotrophic factor biology.protein Receptor |
| Zdroj: | Neurogastroenterology & Motility. 22:971-e252 |
| ISSN: | 1350-1925 |
| Popis: | Background Transient receptor potential vanilloid-1 (TRPV1) receptor has been implicated in the mechanism of acid induced inflammation in gastro-esophageal reflux disease (GERD). It has been demonstrated that the increase in nerve growth factor (NGF) and glial derived neurotrophic factor (GDNF) was associated with the increased expression of TRPV1. We aimed to determine whether expression of TRPV1 was increased in severe inflamed human esophagus, and to test the hypothesis whether the expression of TRPV1 was mediated by neurotrophic factors such as NGF and GDNF. Methods We compared biopsies taken from the distal esophagus of 15 patients with erosive GERD, 16 asymptomatic patients (AP), and 10 healthy controls. We assessed the biopsies with reverse transcription polymerase chain reaction (RT-PCR) and real-time quantitative polymerase chain reaction (qPCR) for TRPV1, NGF, and GDNF. Immunohistochemical analysis of TRPV1 protein expression was also determined. Key Results Transient receptor potential vanilloid-1 mRNA level and its protein expression were significantly greater in patients with erosive esophagitis than AP (P |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|