Novel laccase—producing ascomycetes
| Autor: | Zh. V. Renfeld, Thomas Classen, M. P. Kolomytseva, A.M. Chernykh, Jörg Pietruszka, N. M. Myasoedova, A. S. Samoilova, L. A. Golovleva, E. V. Podieiablonskaia |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Laccase ABTS biology 030106 microbiology Substrate (chemistry) biology.organism_classification Applied Microbiology and Biotechnology Microbiology Agar plate Ferulic acid 03 medical and health sciences chemistry.chemical_compound chemistry Botany Ulocladium atrum Myrothecium roridum Food science Coniferyl alcohol |
| Zdroj: | Microbiology. 86:503-511 |
| ISSN: | 1608-3237 0026-2617 |
| Popis: | Screening of ascomycetes producing laccases during growth on agar medium or submerged cultivation in the presence of various natural sources of carbon and energy (grain crops and potato) was carried out. The conditions of submerged cultivation of the most active strains (Myrothecium roridum VKM F-3565, Stachybotrys cylindrospora VKM F-3049, and Ulocladium atrum VKM F-4302) were optimized for the purpose of increasing laccase activity. The pH-optima and substrate selectivity of laccases in the culture liquid of the strains in relation to ABTS and phenolic compounds (2,6-dimethoxyphenol, syringaldazine, ferulic acid, p-coumaryl alcohol, and coniferyl alcohol) were investigated. High laccase activity at neutral pH was shown for the culture liquids of M. roridum VKM F-3565 and S. cylindrospora VKM F-3049 strains that provides prospects for using laccases of these strains in various cell biotechnologies. |
| Databáze: | OpenAIRE |
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