In vitro response of Ichthyophthirius multifiliis to sera from immune channel catfish
| Autor: | Harry W. Dickerson, J. B. Gratzek, R. C. Findly, Theodore G. Clark |
|---|---|
| Rok vydání: | 1987 |
| Předmět: |
Antiserum
Serial dilution Ichthyophthirius multifiliis animal diseases chemical and pharmacologic phenomena biochemical phenomena metabolism and nutrition Aquatic Science Biology biology.organism_classification Mucus Microbiology Immune system Immunology biology.protein bacteria Antibody Pathogen Ecology Evolution Behavior and Systematics Catfish |
| Zdroj: | Journal of Fish Biology. 31:203-208 |
| ISSN: | 1095-8649 0022-1112 |
| DOI: | 10.1111/j.1095-8649.1987.tb05314.x |
| Popis: | Sera from channel catfish rendered immune to the protozoan pathogen Ichthyophthirius multifiliis were screened for activity against live parasites. Cells in the infective stage (tomites) were incubated in doubling dilutions of immune and pre-immune sera from fish that had been immunized by exposure to sublethal infections. When examined by light microscopy, tomites were found to agglutinate in the presence of immune sera. While the stength of individual sera varied, agglutination of cells occurred at dilutions as high as 1:128. Cells showed little tendency to agglutinate in pre-immune sera, and virtually no effects were seen with dilutions of pre-immune sera greater than 1:16. Agglutination was usually accompanied by release of mucus from cells, and while tomites appeared to be immobilized, their cilia continued to beat. Low dilutions of immune sera appeared to be toxic. Similar effects on tomites were seen with rabbit antisera prepared against Ichthyophthirius cilia. The involvement of humoral antibodies in agglutination and protective immunity is discussed. |
| Databáze: | OpenAIRE |
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