Determination of wound healing activity of Shark liver oil using the excision wound model in Wistar rats
| Autor: | Das S K, Jat R K, Shukla S T, Auti S A |
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| Rok vydání: | 2020 |
| Předmět: |
Contraction (grammar)
biology business.industry Shark liver oil Glutathione Pharmacology Superoxide dismutase chemistry.chemical_compound medicine.anatomical_structure Wound area Dermis chemistry medicine biology.protein General Pharmacology Toxicology and Pharmaceutics Wound healing business Excision wound |
| Zdroj: | International Journal of Research in Pharmaceutical Sciences. 11:7301-7308 |
| ISSN: | 0975-7538 |
| DOI: | 10.26452/ijrps.v11i4.3869 |
| Popis: | There are various promising preclinical models, such as in mice, wistar rats, rabbits and pigs, which can be utilized to initiate acute or chronic wounds. These can be persuaded by many distinctive techniques, with excision the most common. After determining a proper model for a study, investigators need to choose an appropriate and reproducible technique that will permit the monitoring of the wound improvement over time. In this study, the healing power of Shark Liver Oil Emulgel (SLO) in Wistar rats were analyzed by using the excision wound model. The shark liver oil was prepared as emulgel at a concentration of 5%,10% and 15%, respectively and both Standard drug(Povidone-Iodine Ointment USP 5% w/w-PI) and SLO is applied at a concentration of 1mg/mm2; Topically. The parameters integrated for the assessment of the effects of SLO were Relative body weight changes, wound area contraction in mm2, relative wound percentage and epithelialization time. Wound area contraction was measured on 1st, 3rd, 6th, 9th, 12th, 15th, 18th and 21st day. Wistar rats treated with SLO showed substantial variations in epithelialization period and improved wound contraction in the excision wound treatment as compared to disease control. The biochemical biomarkers like SOD(Superoxide dismutases), GSH(Reduced Glutathione)and LPO(Lipid peroxidase) in the treated group have shown a significant change in the improvement of wound healing. Histopathological studies and microscopic observations specify that the topical use of Shark liver oil Emulgel extensively improved wound contraction, collagenation and epithelialization with well-organized dermis devoid of inflammatory cells in contrast to disease control. |
| Databáze: | OpenAIRE |
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