Molecular evolution and phylogenetic implications of internal transcribed spacer sequences of Ribosomal DNA in Winteraceae

Autor:	Leonard B. Thien, Elizabeth A. Zimmer, Youngbae Suh, Helena E. Reeve
Rok vydání:	1993
Předmět:	Genetics Phylogenetic tree Winteraceae Nucleic acid sequence Plant Science Spacer DNA Biology biology.organism_classification Molecular evolution Phylogenetics Internal transcribed spacer Ribosomal DNA Ecology Evolution Behavior and Systematics
Zdroj:	American Journal of Botany. 80:1042-1055
ISSN:	0002-9122
DOI:	10.1002/j.1537-2197.1993.tb15332.x
Popis:	The internal transcribed spacers and the 5.8S coding region of nuclear ribosomal DNA were sequenced and analyzed to address questions of generic relationships in Winteraceae. The molecular data generated a single tree that is congruent with one based on morphological data. The sequences of ITS 1 in the family range from 235 to 252 bases in size and of ITS 2 from 213 to 226 bases. The size of the 5.8S coding region is 164 bases. The range of ITS 1 and ITS 2 sequence divergence between pairs of genera within Winteraceae is relatively low in comparison to other plant families. Two types of ITS 1 and ITS 2 sequences were observed in the same individual for some taxa. Sequence variations between the two arrays are 4.70/-o 6.3% for ITS 1 and 5. 10/o-7.0% for ITS 2. Both arrays of sequences, however, generate the same phylogenetic relationships. Rates of nucleotide substitutions for the internal transcribed spacers are 3.2-5.2 x 10-'? substitution per site per year estimated in ITS 1 and 3.6-5.7 x 10-'? in ITS 2.
Databáze:	OpenAIRE
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