Development of an enzyme-linked immunosorbent assay for detection of circulating IgG autoantibodies against canine desmoglein 3 in dogs with pemphigus

Autor:	Masayuki Amagai, Hitomi Konno, Koji Nishifuji, Thierry Olivry, Kei Tamura, Toshiroh Iwasaki
Rok vydání:	2009
Předmět:	education.field_of_study General Veterinary Screening test business.industry Pemphigus vulgaris Autoantibody medicine.disease Molecular biology Pemphigus Titer Paraneoplastic pemphigus Immunology Desmoglein 3 medicine business education Pemphigus foliaceus
Zdroj:	Veterinary Dermatology. 20:331-337
ISSN:	1365-3164 0959-4493
DOI:	10.1111/j.1365-3164.2009.00806.x
Popis:	Pemphigus, a group of autoimmune blistering dermatoses recognized in humans and dogs, is divided into three subgroups: pemphigus vulgaris (PV), pemphigus foliaceus (PF) and paraneoplastic pemphigus (PNP). Previously, we have demonstrated that circulating anti-desmoglein 3 (Dsg3) IgG in dogs with PV and PNP disrupts Dsg3-mediated keratinocyte cell–cell adhesion. The aim of this study was to develop an enzyme-linked immunosorbent assay (ELISA) for the detection of circulating IgG against canine Dsg3 in dogs with pemphigus. A secreted form of recombinant protein representing the entire extracellular domain of canine Dsg3 (cDsg3) was produced by baculovirus expression and used as the coated antigen in the ELISA. The titre of anti-cDsg3 ELISA was significantly higher in canine PV (n = 14) than that in healthy dogs (n = 44) (P
Databáze:	OpenAIRE
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