| Autor: |
Jianquan Wang, Liwen Zhang, Lei Fu, Linxin Chen, Hongjie Huang, Wenjie Dai, Qing Cai, Xin Zhang, Jiajin Fang, Xiaoping Yang |
| Rok vydání: |
2021 |
| Předmět: |
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| Zdroj: |
Chemical Engineering Journal. 422:130428 |
| ISSN: |
1385-8947 |
| Popis: |
Despite many advanced efforts have been made in osteochondral tissue engineering, it remains challenging to rehabilitate cartilage-to-bone interface complex through three-dimensional (3D) scaffolding treatment. Inspired by extracellular matrix (ECM) fibrous structure and heterogeneous osteochondral interface, herein, a novel 3D fibrous aerogel (gradient aerogel, A-G) with composition and mechanical gradients was designed with three layers based on electrospun poly(L-lactide)/gelatin composite fibers and controlled biomineralization technique. The A-G aerogel, briefly, contained glycosaminoglycan in its top layer and apatite in its middle/bottom layers. In vitro results indicated that this hierarchical aerogel could induce bone marrow mesenchymal stromal cells (BMSCs) to exhibit zone-specific morphology and bi-directionally differentiate into chondrogenic or osteogenic phenotypes. A cell affinity peptide, E7-peptide, was grafted onto the A-G aerogel to endow it with improved capacity in enhancing cell migration and recruiting BMSCs for in vivo applications. The E7-conjugated gradient aerogel (A-E7G) was implanted into an osteochondral defect (4 mm × 4 mm) created in rabbit knee for 12 weeks. Compared to the aerogel without composition gradient and the A-G aerogel, the A-E7G aerogel reconstructed the osteochondral interfacial tissue with hierarchically mature structure and clear tidemark more efficiently in an enhanced mode. These results indicated that the E7-peptide modified gradient aerogel could be a promising candidate in the osteochondral tissue engineering in the way of no growth factor addition. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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