Biogenic synthesis of silver nanoparticles using cell-free extract of Bacillus safensis LAU 13: antimicrobial, free radical scavenging and larvicidal activities
| Autor: | Lorika S. Beukes, Abiola S. Akinwale, Agbaje Lateef, Sunday A. Ojo, Evariste B. Gueguim-Kana, Luqmon Azeez |
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| Rok vydání: | 2015 |
| Předmět: |
Bacillus safensis
biology Chemistry technology industry and agriculture Aspergillus flavus Cell Biology Plant Science Antimicrobial medicine.disease_cause biology.organism_classification Biochemistry Silver nanoparticle Aspergillus fumigatus Staphylococcus aureus Genetics medicine Animal Science and Zoology Ofloxacin Molecular Biology Escherichia coli Ecology Evolution Behavior and Systematics medicine.drug Nuclear chemistry |
| Zdroj: | Biologia. 70:1295-1306 |
| ISSN: | 1336-9563 0006-3088 |
| DOI: | 10.1515/biolog-2015-0164 |
| Popis: | The cell-free extract of Bacillus safensis LAU 13 strain (GenBank accession No: KJ461434) was used for green biosynthesis of silver nanoparticles (Ag-NPs). Characterization of Ag-NPs was carried out using UV-VIS spectroscopy, Fourier-transform infrared spectroscopy, and transmission electron microscopy. Evaluation of synthesized Ag-NPs as antimicrobial agents was done using multi-drug resistant clinical isolates as well as their synergistic effects when combined with some selected antibiotics. Furthermore, potential of Ag-NPs as antimicrobial additives in paint was demonstrated. The Ag-NPs have maximum absorbance at 419 nm, with peaks at 3308, 2359, 1636, and 422 cm-1, indicating that proteins were the capping and stabilisation molecules in the synthesis of Ag-NPs. The particles were spherical shaped having size of 5–95 nm, with silver as the prominent metal from the energy dispersive X-ray analysis, while selected area electron diffraction pattern agrees well with the crystalline nature and face-centred cubic phase of Ag-NPs. Inhibition of Staphylococcus aureus, Escherichia coli, Klebsiella granulomatis and Pseudomonas aeruginosa was achieved at 100 μg/mL. Improvement of activities of augmentin, ofloxacin and cefixime to the tune of 7.4-142.9% was achieved in synergistic study, while total inhibitions of P. aeruginosa, S. aureus, Aspergillus flavus and Aspergillus fumigatus were achieved in Ag-NPs-paint admixture. The Ag-NPs showed potent antioxidant and larvicidal activities with IC50 and LC50 of 15.99 and 42.19 μg/mL, respectively. The present study demonstrated that the biosynthesized Ag-NPs have potent biological activities, which can find applications in diverse areas. The report adds to the growing biotechnological relevance of B. safensis. |
| Databáze: | OpenAIRE |
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