APC promoter hypermethylation contributes to the loss of APC expression in colorectal cancers with allelic loss on 5q1
| Autor: | Linda Wasserman, Christian N. Arnold, C. Richard Boland, Jeannette M. Dowell, Monica M. Bertagnolli, Ajay Goel, Donna Niedzwiecki, Carolyn C. Compton, Robert J. Mayer |
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| Rok vydání: | 2004 |
| Předmět: | |
| Zdroj: | Cancer Biology & Therapy. 3:960-964 |
| ISSN: | 1555-8576 1538-4047 |
| DOI: | 10.4161/cbt.3.10.1113 |
| Popis: | Introduction: Germ-line mutations of the APC gene are associated with familial adenomatous polyposis, and somatic mutations occur frequently in sporadic colorectal cancer. However, to abrogate APC function, both alleles must be inactivated. Recently, it has been demonstrated that epigenetic modification of the APC promoter influences APC silencing. Here we examined the influence of APC methylation on APC expression in tumors with and without LOH at the APC locus. Material and Methods: 137 sporadic colorectal cancer specimens were investigated for LOH at the 5q locus. The methylation status of the APC promoter was determined by methylation-specific PCR. APC expression was performed by immunohistochemistry. Results: APC expression was reduced or lost in 110 of 137 (80%) tumors and LOH at 5q was found in 13 of 132 (10%) tumors. There was no difference in 5q LOH between tumors with or without intact APC expression. Vice versa, there was no difference in the APC expression in tumors with 5q LOH. Aberrant APC p... |
| Databáze: | OpenAIRE |
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