Molecular analysis of five rice blast resistant genes in landraces from Myanmar and Laos
| Autor: | Ma Jiqiong, Cuifeng Tang, Xinxiang A, Yang Yi, Sun Yiding, Xu Minghui |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
0301 basic medicine Veterinary medicine Oryza sativa biology Haplotype Plant Science biology.organism_classification 01 natural sciences Molecular analysis 03 medical and health sciences 030104 developmental biology Genetic marker Genotype Genetics Magnaporthe grisea Resistant genes Agronomy and Crop Science Gene 010606 plant biology & botany |
| Zdroj: | Plant Genetic Resources: Characterization and Utilization. 18:287-293 |
| ISSN: | 1479-263X 1479-2621 |
| DOI: | 10.1017/s1479262120000271 |
| Popis: | In order to understand rice blast resistance gene patterns in rice landraces from Myanmar and Laos, we analysed thePita,Pib,Pikh,Pi9andPi5genes in 80 landraces through the use of functional molecular markers and functional fragment sequencing. These landraces were separated into four haplotypes (H1–H4) based upon three mutations identified in thePitasequence. All four haplotypes were detected among landraces from Myanmar, whereas only the H1 and H2 haplotypes were detected among landraces from Laos. We additionally grouped landraces carrying 0–4 resistance genes into 18 genotypes based upon their resistance-susceptibility patterns and found that 16 of these genotypes were detected among Myanmar landraces at relative frequencies ranging from 2.50–12.50%, while 10 were detected among landraces from Laos at relative frequencies of 2.50–42.50%. The ‘Pib( + )pikh(−)pi9(−)pi5(−)pita(−)’ genotype was found to be dominant, accounting for 12.5 and 42.5% of landraces from Myanmar and Laos, respectively. ThePib,Pikh,Pi5andPitagenes were detected in 52.50, 32.50, 37.50 and 30.00% of landraces from Myanmar respectively, whereas they were detected in 52.50, 12.50, 30.00 and 17.50% of landraces from Laos, respectively. ThePi9gene was rarely detected among analysed landraces and was particularly rare in those from Laos. These findings suggest that rice landraces from Myanmar are more genetically diverse than those from Laos, with clear differences in blast resistance gene patterns between landraces from these two countries. |
| Databáze: | OpenAIRE |
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