Reaction Conditions for Maximal Cyclodextrin Production by Cyclodextrin Glucanotransferase from Bacillus megaterium
| Autor: | Veselin Stanchev, Boriana Zhekova |
|---|---|
| Rok vydání: | 2011 |
| Předmět: |
Microbiology (medical)
chemistry.chemical_classification Reaction conditions Chromatography Cyclodextrin biology Starch Substrate (chemistry) General Medicine biology.organism_classification Applied Microbiology and Biotechnology Microbiology Substrate concentration chemistry.chemical_compound Enzyme chemistry Biochemistry Modelling methods Bacillus megaterium |
| Zdroj: | Polish Journal of Microbiology. 60:113-118 |
| ISSN: | 2544-4646 1733-1331 |
| Popis: | The effect of the reaction conditions (substrate concentration, enzyme dosage, and pH) on cyclodextrin production by cyclodextrin glucanotransferase from Bacillus megaterium was investigated by applying mathematical modeling methods. Adequate models were developed and they were used for determination of the optimal conditions for maximal formation of beta-cyclodextrins at minimal concentrations of a- and gamma-cydclodextrins. The main factor affecting the ratio of the products was pH of the reaction mixture. At pH 9 the enzyme formed mainly beta- and y-cyclodextrins and the ratio a:beta:gamma was 2.6:83.5:13.9; at pH 5 the ratio changed to 8.6:84.6:6.8. Mathematical models were used for determination of the conditions for maximal conversion of the substrate into cyclodextrins. 45.88% conversion of starch was achieved at 5% substrate concentration, 3.5 U/g enzyme dosage, and pH 7.4. |
| Databáze: | OpenAIRE |
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