A new morphometric technique for assessment of melanization in skin of guinea pigs
| Autor: | Amal T. Abdel-Rahman, Ahmed Fawzy, Tag S. Anbar, Manal Barakat, Tarek S. El-Ammawi |
|---|---|
| Rok vydání: | 2011 |
| Předmět: |
Pathology
medicine.medical_specialty integumentary system medicine.diagnostic_test Immunology Significant difference H&E stain Objective measurement Dermatology General Medicine Biology Melanin Narrow band Biopsy medicine Immunology and Allergy Radiology Nuclear Medicine and imaging Statistical analysis |
| Zdroj: | Photodermatology, Photoimmunology & Photomedicine. 28:42-46 |
| ISSN: | 0905-4383 |
| DOI: | 10.1111/j.1600-0781.2011.00636.x |
| Popis: | Summary Background The degree of melanization is an important finding in many skin disorders. An objective measurement of melanin density is needed to achieve an accurate evaluation. Objectives The present work aims at translating the subjective increase of melanin particles after narrow band ultraviolet-B (NB-UVB) irradiation into objective numerical values ready for statistical analysis. Materials and Methods This study had involved 18 guinea pigs that were exposed to biweekly sessions of NB-UVB radiation for 4 weeks to induce skin pigmentation. Two skin biopsies were obtained from each animal; the first before treatment and the second at the end of the study, using 5 mm punch and stained with hematoxylin and eosin and Masson–Fontana (MF) stains. Surface area of both the epidermis (ESA) and the melanin particles (MPSA) were measured in μm2 using a software supplied with Olympus light microscope. The MPSA/ESA percentage was calculated for each biopsy. The results before and after NB-UVB exposure were compared and statistically analyzed. Results In the MF-stained sections, the mean ± SD of the MPSA/ESA percentage were 0.24 μm2 ± 0.09 and 6.21 μm2 ± 2.45 at the start of the study and at its end, respectively, with a highly significant difference (P |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text