24-Hour Presumptive Enumeration of Escherichia coli O157:H7 in Foods by Using the ISO-GRID® Method with SD-39 Agar
| Autor: | Phyllis Entis, Irina Lerner |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
food.ingredient
biology food and beverages Pasteurization medicine.disease_cause biology.organism_classification Microbiology Enterobacteriaceae law.invention chemistry.chemical_compound food chemistry Most probable number law Tryptone medicine Enumeration Agar Food science Escherichia coli Bacteria Food Science |
| Zdroj: | Journal of Food Protection. 60:883-890 |
| ISSN: | 0362-028X |
| DOI: | 10.4315/0362-028x-60.8.883 |
| Popis: | Two 24-hour presumptive enumeration methods for Escherichia coli O157:H7 organisms based on the hydrophobic grid membrane filter (ISO-GRID) and using SD-39 agar, a new selective and differential culture medium, were developed and compared to a 3-tube MPN (most probable number) method using modified tryptone soy broth enrichment. The comparative study comprised 22 combinations of storage conditions and food products, including a variety of raw and cooked meats and several dairy products. The ISO-GRID direct filtration method produced counts which were equivalent to or significantly higher than the 3-tube MPN method for all food-storage combinations except for frozen pasteurized whole egg. The ISO-GRID resuscitation method produced counts equivalent to the 3-tube MPN method for the frozen egg. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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