Effect of nutrient media on axillary shoot proliferation and preconditioning for adventitious shoot regeneration of pears

Autor:	Chinnathambi Srinivasan, Delores Lomberk, Richard L. Bell
Rok vydání:	2009
Předmět:	PEAR Chlorosis biology Plant tissue culture fungi Clinical Biochemistry food and beverages Plant Science Cell Biology biology.organism_classification Basal shoot Micropropagation Shoot Botany Biotechnology Developmental Biology Pyrus communis Explant culture
Zdroj:	In Vitro Cellular & Developmental Biology - Plant. 45:708-714
ISSN:	1475-2689 1054-5476
DOI:	10.1007/s11627-009-9196-8
Popis:	The influence of the nutrient composition of plant tissue culture media on axillary shoot proliferation and their preconditioning effect on subsequent adventitious shoot regeneration from pear leaves was investigated. The goal was to improve both micropropagation and regeneration of ‘Bartlett’ and ‘Beurre Bosc’ pear cultivars. Driver–Kuniyuki walnut (DKW) and Quoirin and Lepoivre (QL) nutrient media were found to be superior to Murashige and Skoog (MS) and Woody Plant Medium (WPM) for axillary shoot proliferation. Shoots on WPM exhibited some chlorosis. Axillary shoot culture on DKW would be preferred to that on QL due to the production of excessively short thin shoots on the latter medium. DKW also was superior to QL and MS for production of young expanding leaves for use as explants in adventitious regeneration. Leaf explants derived from shoot proliferation cultures grown on DKW or QL media produced more adventitious shoots than leaf explants from MS.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::dfe59fb3ac308731577d518b6bb000bb https://doi.org/10.1007/s11627-009-9196-8 Zobrazit plný text záznamu Full text from SpringerLink