Progesterone Plays a Critical Role in Canine Oocyte Maturation and Fertilization1
| Autor: | Sandra Thoumire, Karine Reynaud, Marie Saint-Dizier, Valérie Labas, Grégoire Harichaux, Tiphaine Havard, Bénédicte Grimard, Alain Fontbonne, Sylvie Chastant-Maillard, M. Z. Tahir |
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| Rok vydání: | 2015 |
| Předmět: |
endocrine system
medicine.medical_specialty media_common.quotation_subject Uterus Biology 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Aglepristone Human fertilization Internal medicine medicine Ovulation Sperm motility 030304 developmental biology media_common 0303 health sciences 030219 obstetrics & reproductive medicine Embryogenesis Cell Biology General Medicine Oocyte Sperm 3. Good health medicine.anatomical_structure Endocrinology Reproductive Medicine chemistry |
| Zdroj: | Biology of Reproduction. 93 |
| ISSN: | 1529-7268 0006-3363 |
| DOI: | 10.1095/biolreprod.115.130955 |
| Popis: | Canine oocyte maturation and fertilization take place within the oviducts under increasing plasma levels of progesterone (P4). In order to investigate the role of P4 in these processes, 51 beagle bitches were treated with the P4 receptor antagonist aglepristone at the end of proestrus and 32 females were kept untreated. Fifteen treated and 13 control bitches were inseminated at Days +1 and +2 after ovulation (Day 0). Stages of oocyte maturation and embryo development were determined after ovariectomy at different time points after ovulation. Aglepristone did not prevent ovulation but delayed the resumption of oocyte meiosis and inhibited its progression: first metaphase I (MI) stage was observed at 173 h postovulation and 39% of oocytes reached MII as late as 335 h postovulation in treated females whereas first MI occurred at 76 h and 100% of oocytes were in MII at 109 h postovulation in controls. Aglepristone extended the stay of morphologically normal oocytes within the oviducts: first signs of oocyte degeneration were observed at 335 h in treated versus 100- to 110-h postovulation in control bitches. In inseminated females, aglepristone prevented sperm progression toward the oviducts and fertilization, although motile spermatozoa were observed in the uterine tip flush and within the cranial uterine glands. A proteomic analysis of the tubal fluid from treated and control noninseminated bitches at Day +4 found evidence of 79 differential proteins potentially involved in the oocyte phenotype. In conclusion, P4 plays key roles in postovulatory canine oocyte maturation, aging, and in fertilization. |
| Databáze: | OpenAIRE |
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