Synthesis of a conjugate of 3´-sialyllactoside with recombinant flagellin as a carrier protein and assessment of its immunological activity in comparison with that of a similar hemocyanin-based conjugate
| Autor: | O. A. Zeynalov, Yu. E. Tsvetkov, D. A. Goncharuk, R. N. Stepanenko, E. M. Novikova, Nikolay E. Nifantiev, N. A. Razvalyaeva, Elena A. Khatuntseva |
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| Rok vydání: | 2015 |
| Předmět: |
0301 basic medicine
biology Chemistry medicine.medical_treatment Hemocyanin General Chemistry Dendritic cell Molecular biology law.invention 03 medical and health sciences 030104 developmental biology 0302 clinical medicine Antigen law 030220 oncology & carcinogenesis Recombinant DNA biology.protein medicine Cancer vaccine Antibody Flagellin Conjugate |
| Zdroj: | Russian Chemical Bulletin. 64:1640-1647 |
| ISSN: | 1573-9171 1066-5285 |
| Popis: | In the framework of a project directed to the design of synthetic cancer vaccines based on carbohydrate chains of tumor-associated gangliosides, a new prototype cancer vaccine, a polyvalent carbohydrate-protein conjugate 1 with ∼11 3´-sialyllactoside ligands conjugated to one molecule of recombinant flagellin was synthesized. Dendritic cell vaccines were designed using the conjugate 1 and a neoglycoconjugate 2 consisting of ∼400 3´-sialyllactose residues linked to hemocyanin from a snail Megathura crenulata (KLH). Comparative ELISA study was conducted to assess the induction of carbohydrate-specific immunoglobulins IgM and IgG after immunization of mice with conjugates 1 and 2 and dendritic cells loaded with 1 and 2. Synthetic polyacrylamide carrier conjugated to 3´-sialyllactose ligands and B16F0 melanoma cells were used as coating antigens. |
| Databáze: | OpenAIRE |
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