IgE-mediated facilitated antigen presentation underlies higher immune responses in peanut allergy
| Autor: | Alick Stephens, F. Rancé, Susan M H Chan, Victor Turcanu, Gideon Lack |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
biology
business.industry Immunology Antigen presentation Peanut allergy food and beverages Carboxyfluorescein succinimidyl ester Immunoglobulin E medicine.disease Peripheral blood mononuclear cell chemistry.chemical_compound Immune system chemistry Antigen biology.protein medicine Immunology and Allergy Antibody business |
| Zdroj: | Allergy. 65:1274-1281 |
| ISSN: | 0105-4538 |
| DOI: | 10.1111/j.1398-9995.2010.02367.x |
| Popis: | Background: Peanut allergy poses significant healthcare problems, because its prevalence is increasing in many countries, and it is rarely outgrown. To explore the immunological mechanisms that underlie peanut allergy and tolerance, we compared the peanut-specific responses of peanut-allergic (PA) and nonallergic (NA) individuals. Methods: We measured peanut-specific peripheral blood mononuclear cells (PBMC) proliferation using tritiated thymidine. The frequency of peanut-specific T cells amongst PBMC was determined by carboxyfluorescein succinimidyl ester labelling. The role of IgE-dependent facilitated antigen presentation (FAP) in modulating proliferation was investigated by depleting IgE from plasma with anti-IgE-coated beads and then assessing PBMC proliferation in the presence of IgE-depleted or nondepleted plasma. Results: We found that peanut-specific PBMC proliferation is higher and peaks earlier in PA than in NA donors. We investigated the immunological mechanisms that could underlie these differences. We found that both PA and NA have memory responses to peanut, but the frequency of peanut-specific T cells is higher in PA than in NA. Facilitated antigen presentation could cause both the higher proliferation and precursor frequency in PA. Facilitated antigen presentation activity in vitro was confirmed by showing that IgE depletion decreases proliferation, while adding IgE back restores it. Conclusion: Our results identify FAP as a mechanism that underlies higher responses to peanut in PA. In these individuals, high levels of peanut-specific IgE could furthermore maintain long-term allergic T-cell responses. We raise the question whether, in the future, therapies targeting IgE such as anti-IgE antibodies may be used to suppress these T-cell responses. |
| Databáze: | OpenAIRE |
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