Cocaine- and amphetamine-regulated transcript (CART) peptide immunoreactivity in myenteric plexus neurons of the rat ileum and co-localization with choline acetyltransferase
| Autor: | Yoland Smith, Pastor R. Couceyro, Maryse Paquet, Ersin O. Koylu, Michael J. Kuhar |
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| Rok vydání: | 1998 |
| Předmět: |
Cart
medicine.medical_specialty virus diseases Neuropeptide Biology Choline acetyltransferase Molecular biology Cocaine and amphetamine regulated transcript Cellular and Molecular Neuroscience chemistry.chemical_compound medicine.anatomical_structure Endocrinology nervous system chemistry immune system diseases Internal medicine parasitic diseases mental disorders medicine Axon Neurotransmitter Acetylcholine Myenteric plexus medicine.drug |
| Zdroj: | Synapse. 30:1-8 |
| ISSN: | 1098-2396 0887-4476 |
| DOI: | 10.1002/(sici)1098-2396(199809)30:1<1::aid-syn1>3.0.co;2-7 |
| Popis: | Cocaine and amphetamine regulated transcript (CART) encodes a novel brain-enriched protein whose features are reminiscent of a neurotransmitter propeptide. We have now localized CART peptide(s) in the gastrointestinal tract by immunohisto- chemical methods. Polyclonal antisera raised to CART peptide 106-129 stained neuronal cell bodies and fibers in rat ileum myenteric plexus-longitudinal muscle tissue prepara- tions. Electron microscopic analysis of thin sections showed immunopositive axon terminals in close apposition to CART-labelled and unlabelled neuronal cell bodies as well as to the longitudinal muscle. CART peptide-immunoreactive terminals contained numerous ovoid electron-lucent vesicles and a few dark-stained dense-core vesicles. Light microscopic double labelling studies revealed CART peptide immunoreactivity in a subpopulation of choline acetyltransferase (ChAT)-immunoreactive neurons. This com- bined light microscopic and ultrastructural examination of CART peptides in the gastrointestinal tract suggests a role of CART peptides as transmitters or neuromodula |
| Databáze: | OpenAIRE |
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