Isocitrate lyase activity in halophilic archaea

Autor:	Peter Gurevich, Aharon Oren
Rok vydání:	1995
Předmět:	Haloferax volcanii Glyoxylate cycle Isocitrate lyase Biology Lyase biology.organism_classification Microbiology Haloferax mediterranei Biochemistry Genetics Isocitrate lyase activity Haloferax Molecular Biology Magnesium ion
Zdroj:	FEMS Microbiology Letters. 130:91-95
ISSN:	1574-6968 0378-1097
Popis:	Eight species of halophilic Archaea were tested for the presence of isocitrate lyase activity. High activities (up to 100 nmol min−1 mg protein−1) were detected in Haloferax mediterranei and Haloferax volcanii when grown in medium containing acetate as the principal carbon source. Little activity was found in representatives of the genera Halobacterium and Haloarcula. Isocitrate lyase from Haloferax mediterranei required high potassium chloride concentrations, optimal activity being found at 1.5–3 M potassium chloride and pH 7.0. Replacement of potassium chloride by sodium chloride resulted in much lower activities. Sulfhydryl compounds (cysteine, glutathione) were not stimulatory. In other properties (stimulation by magnesium ions, sensitivity to different inhibitors) the enzyme resembled isocitrate lyases from representatives of the Bacteria and Eucarya.
Databáze:	OpenAIRE
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