Identification of coccidioidomycosis causative agent by means of polymerase chain reaction

Autor:	Antonov Va, Zamaraev Vs, Grishina Ma, G. A. Tkachenko, A. V. Lipnitskii, S. S. Savchenko, V. S. Lesovoi
Rok vydání:	2007
Předmět:	Coccidioides species Biology biology.organism_classification Microbiology DNA extraction Virology law.invention Highly sensitive Infectious Diseases law Genetics Identification (biology) Coccidioides Molecular Biology Gene Polymerase chain reaction Specific identification
Zdroj:	Molecular Genetics, Microbiology and Virology. 22:165-171
ISSN:	1934-841X 0891-4168
DOI:	10.3103/s0891416807040052
Popis:	For the purpose of diagnosing coccidioidomycosis by means of PCR, two pairs of primers were constructed. The first one was developed on the basis of MBP-I gene to be used for identification of two Coccidioides species, C. immitis and C. posadasii. The other pair of primers was constructed on the basis of the gene encoding immunodominant glycoprotein of the spherule outer wall (SOW gp82) to be used for identification of C. posadasii. With these primers, both highly sensitive and highly specific identification of coccidioidomycosis causative agents proved to be possible. An efficient procedure was developed for isolation of fungal DNA from soil contaminated with Coccidioides spp. arthrospores. This procedure includes guanidine thiocyanate-phenol-chloroform deproteinization followed by DNA purification by means of nucleosorption.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::df7a31a072cb572556c10f73a83ec936 https://doi.org/10.3103/s0891416807040052 Zobrazit plný text záznamu Full text from SpringerLink