Expression Profile of GINS Complex Predicts the Prognosis of Pancreatic Cancer Patients

Autor: Xuan Yi, Chen Luo, Chao Huang, Ce-Gui Hu, Zitao Liu, Jiefeng Zhao, Jun Huang, Kang Lin, Xiaojian Zhu, Wen-jun Zhang, Fanqin Bu, Jinfeng Zhu
Rok vydání: 2020
Předmět:
Zdroj: OncoTargets and Therapy. 13:11433-11444
ISSN: 1178-6930
DOI: 10.2147/ott.s275649
Popis: Background The GINS complex has been implicated in the prognosis of various cancers. It comprises four subunits, encoded by GINS1, GINS2, GINS3, and GINS4 genes. Based on the current understanding, no report exists on the role of the GINS complex in pancreatic cancer. Methods We employed various bioinformatics databases including GEPIA, UALCAN, GEPIA2, and Kaplan Meier Plotter to identify the expression profile of the four genes (GINS1, GINS2, GINS3, and GINS4), their correlation with pancreatic cancer grade as well as their prognostic value of in pancreatic cancer. Western blotting and qRT-PCR analyses were conducted to verify the expression profiles of the four genes in pancreatic cancer. CCK8 and EdU cell experiments were conducted to reveal the role played by the four genes in pancreatic cancer cell proliferation. Results Based on GEPIA, Western blotting, and qRT-PCR analyses, all the four genes in the GINS complex were overexpressed in pancreatic cancer. Notably, the expression of each member was significantly associated with pancreatic cancer grade. The prognostic analysis revealed that not only the whole GINS complex but also each individual were prognostic biomarkers for pancreatic cancer. CCK8 and EdU experiments demonstrated that inhibition of the expression of each GINS member lowered pancreatic cancer cell proliferation. Conclusion This work implicated GINS1, GINS2, GINS3, and GINS4 genes as critical prognostic markers for pancreatic cancer.
Databáze: OpenAIRE
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