Cytotoxicity and cellular uptake of perfluorodecanoic acid
| Autor: | Maria Carmelita Z. Kasuya, Kenichi Hatanaka |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
biology
010405 organic chemistry Chemistry Organic Chemistry Cell Decanoic acid 010402 general chemistry biology.organism_classification 01 natural sciences Biochemistry Molecular biology 0104 chemical sciences Inorganic Chemistry HeLa Melanin chemistry.chemical_compound medicine.anatomical_structure Cell culture medicine Environmental Chemistry Secretion Physical and Theoretical Chemistry Fibroblast Cytotoxicity |
| Zdroj: | Journal of Fluorine Chemistry. 221:56-60 |
| ISSN: | 0022-1139 |
| DOI: | 10.1016/j.jfluchem.2019.03.002 |
| Popis: | Perfluorodecanoic acid (C10F) is a persistent organic pollutant that bioaccumulates. This study investigated the cytotoxicity and cellular uptake of C10F. When compared with the non-fluorinated counterpart, decanoic acid (C10H), results showed that C10F was more toxic to mouse melanoma B16 cells. Whereas a viability of 97% has been observed even up to 800 μM in the presence of C10H, cytotoxicity was already detected at 100 μM (54% viability) in the presence of C10F. Significantly, cellular uptake of C10F was 32-fold higher than that of C10H. Time course analysis showed a correlation between cellular uptake of C10F and cell behavior. After 1 h of incubation in 50 μM C10F, results confirmed that cellular uptake of C10F already occurred concurrent to loss of cell spreading. This was accompanied by secretion of the black pigment melanin commensurate to the level of stress inflicted on the cells caused by the influx of C10F. When the amount of uptake reached a plateau, no significant change in cell behavior was likewise observed up to 24 h. The effect of C10F was also investigated in various human and animal cell lines. The results confirmed the detrimental effect that C10F has on morphology of different cells in culture, and the differences in cellular uptake. In Madin Darby canine kidney MDCK cells and human fetal lung fibroblast TIG cells, 3.75 and 3.42 pg/cell of C10F were detected, respectively. Comparing the human carcinoma cell lines used, 1.1 pg/cell of C10F was detected in hepatocellular (HepG2) and in renal (RCC) cells while 0.87 pg/cell in cervical (HeLa) cells. |
| Databáze: | OpenAIRE |
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