Changes in Tonsil B Cell Phenotypes and EBV Receptor Expression in Children Under 5-Years-Old

Autor: Rosemary Rochford, Anthony J. Mortelliti, Carrie B. Coleman, Eric M. Wohlford, Paul C. Baresel, Joel R. Wilmore
Rok vydání: 2017
Předmět:
Zdroj: Cytometry Part B: Clinical Cytometry. 94:291-301
ISSN: 1552-4949
Popis: Background Palatine tonsils are principally B cell organs that are the initial line of defense against many oral pathogens, as well as the site of infection for others. While the size of palatine tonsils changes greatly in the first five years of life, the cellular changes during this period are not well studied. Epstein Barr virus (EBV) is a common orally transmitted virus that infects tonsillar B cells. Naive B cells are thought to be the target of primary infection with EBV in vivo, suggesting that they are targeted by the virus. EBV enters B cells through CD21, but studies of older children and adults have not shown differences in surface CD21 between naive B cells and other tonsil B cell populations. Methods In this study, we used an 11-color flow cytometry panel to detail the changes in B cell subpopulations in human tonsils over the first five years of life from 33 healthy US children. Results We provide reference ranges for tonsil B cell subpopulations over this age range. We show that the frequency of naive tonsil B cells decreases over the early years of life, and that naive B cells expressed higher surface levels of CD21 relative to other tonsil B cell populations. Conclusions We show that young children have a higher frequency of naive tonsil B cells, and importantly that these cells express increased surface EBV receptor, suggesting that young children have a larger pool of cells that can be infected by the virus. © 2017 International Clinical Cytometry Society.
Databáze: OpenAIRE
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