Enzyme delivery using protein-stabilizing and cell-penetrating 30Kc19α protein nanoparticles
| Autor: | Hee Ho Park, Tai Hyun Park, Hong Jai Lee, Jina Ryu, Ju Hyun Park, Yeon Hwa Woo |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
chemistry.chemical_classification Biomolecule Cell Nanoparticle Bioengineering 02 engineering and technology 021001 nanoscience & nanotechnology Human serum albumin Applied Microbiology and Biotechnology Biochemistry law.invention 03 medical and health sciences 030104 developmental biology medicine.anatomical_structure Enzyme chemistry law Drug delivery medicine Recombinant DNA Specific activity 0210 nano-technology medicine.drug |
| Zdroj: | Process Biochemistry. 63:76-83 |
| ISSN: | 1359-5113 |
| DOI: | 10.1016/j.procbio.2017.08.021 |
| Popis: | Nanoparticles (NPs) are an emerging strategy for drug delivery and have been studied for the delivery of various biomolecules, such as chemically synthesized drugs and therapeutic proteins. In particular, protein NPs are non-cytotoxic and biodegradable. Application of a full length recombinant 30Kc19 protein to human serum albumin (HSA) NPs has been shown to improve the cellular uptake and stability of the cargo enzyme. In this study, we demonstrate that drug delivery can be achieved with only the α-helix domain of the 30Kc19 protein (30Kc19α), and without the addition of HSA. Protein concentration and pH were crucial for NP generation. NPs had a uniformly spherical shape with an optimal diameter of 180–230 nm, and released β-galactosidase in a sustained manner. The 30Kc19α protein provided stability to the cargo enzyme, and helped maintain the specific activity of the enzyme. X-gal staining showed effective delivery of β-galactosidase into human dermal fibroblasts. Non-cytotoxic property of the 30Kc19α protein demonstrates that such NPs could be a resourceful tool for delivering drugs to cells. |
| Databáze: | OpenAIRE |
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