| Autor: |
Dennis J. Crouch, David M. Andrenyak, Matthew L. Cheever, David J. Kuntz, David L. Loughmiller |
| Rok vydání: |
1998 |
| Předmět: |
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| Zdroj: |
Journal of Forensic Sciences. 43:16086J |
| ISSN: |
0022-1198 |
| Popis: |
This study was designed to compare results obtained from two separate on-site drug testing kits (ONTRAK TESTCUP and Abuscreen ONTRAK) with those obtained from laboratory based immunoassay and GC/MS. Abuscreen ONLINE immunoassay was used to select 250 negative samples and 100 presumptive-positive samples each for cocaine/metabolites, opiates and cannabinoids. Presumptive-positive samples were selected if the immunoassay response was ⩾300 ng/mL for cocaine/metabolites (BZE), ⩾300 ng/mL for opiates or ⩾50 ng/mL for cannabinoids (THC-COOH). GC/MS was used to confirm that each selected sample contained ⩾150 ng/mL BZE, ⩾300 ng/mL morphine/codeine or ⩾15 ng/mL THC-COOH. TESTCUP results had a 100% agreement with GC/MS and a >99% agreement with ONLINE when testing negative samples. The agreement between TESTCUP and ONLINE results for samples containing opiates was 100%. Results of testing samples containing BZE with TESTCUP demonstrated a 98% agreement with both GC/MS and ONLINE. Both discrepant samples contained BZE at concentrations ⩽300 ng/mL. The least agreement between TESTCUP and ONLINE results was found when testing samples containing THC-COOH. The agreement with ONLINE and GC/MS was 92% and all discrepant samples had GC/MS determined THC-COOH concentrations less than 50 ng/mL. A 100% agreement was obtained between expected and recorded TESTCUP results for QC samples fortified to contained BZE, morphine or THC-COOH at concentrations within 120% of the screening cutoffs. ONTRAK had a 100% agreement with both GC/MS and ONLINE when testing negative samples and samples that contained opiates. ONTRAK had a 91% agreement with GC/MS and ONLINE for testing of samples that contained BZE. The least agreement between ONTRAK and ONLINE results was found when testing samples that contained THC-COOH. The agreement was 89%, however, all discrepant samples contained GC/MS concentrations of THC-COOH less that the 50 ng/mL cutoff. With ONTRAK, a 100% agreement was obtained between expected and recorded results on QC samples that contained morphine or THC-COOH and a 97.7% agreement was obtained between expected and recorded results on QC samples that contained BZE. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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